Yeast Inoculum Calculator

Email: service@fermaxiom.com

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The web Yeast Inoculum Calculator Basic version is based on direct relationships of the commercial yeast products such as Active Dry Yeast (ADY) or Cream Yeast (CmY) also known as liquid yeast and ethanol fermentation initial yeast inoculum density and process scale. The basic version on this web page is an example of the most basic calculations routinely required to ascertain the usage levels of commercial products. A much more realistic version of the Yeast Inoculum Calculator Advanced is available to our industrial and research partners. The advanced version of the Yeast Inoculum Calculator takes into account variables associated with ethanol fermentation kinetics as well as advanced yeast propagation technologies such as High-Density Yeast Cultivation (HDYC) allowing for functional control of yeast growth and phenotypic characteristics advantages for ethanol fermentation.

Yeast Inoculum Calculator Basic

Standard DYE - Dry Yeast Equivalent (cells/g) Yeast Product Format Total Yeast Cells (cells/g)

Ethanol Fermentation

Yeast Inoculum Target (cells/ml) Fermenter Volume (Physical) [L, gal (US), m³] Fermenter Working Volume (%) Fermenter Working Volume (L) Calculated Direct Pitch Yeast Amount (kg DYE)

YEAST INOCULUM CALCULATOR ADVANCED

Propagation of yeast biomass as the source of inoculum for ethanol fermentation process has a critical impact on overall process yields and process efficiency. While batch yeast propagation at low density operations are the standards typically associated with high gravity Simultaneous Saccharification and Fermentation processes it is of critical importance to ascertain yeast performance economic impact and sustainability of ethanol manufacturing in challenging commodity economy. FermAxiom LLC has developed tools and practices that allow quantification, validation and optimization of yeast performance and strain and technology options available for the industrial ethanol manufacturers. To enhance process quantification and understanding of scientific principles as well as data interpretation the web enabled calculators are provided.

Expected Product Yields & Quantifications for Yeast Propagation:

Yield of Biomass on Glucose Basis (mass to mass in grams)

Y _X/Gluc = 0.1 - 0.5 g/g

Yield of Ethanol on Glucose Basis (mass to mass in grams)

Y _EtOH/Gluc = 0.1 - 0.5 g/g

Yeast Generation Number

Z = 5-10 generations

Yeast Specific Growth Rate

µ = 0.05 – 0.5

Yeast Metabolic Quotients CO₂

qCO₂

Yeast Metabolic Quotients Glycerol

qGlycerol

Yeast Metabolic Quotients Ethanol

qEthanol

In addition to process yields the yeast biomass process kinetics calculations are also performed.