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UPSTREAM BIOPROCESSING

All fermentation dependent manufacturing operations are initialized through Upstream Bioprocessing operations. In the production of ethanol from grain such as corn, the stages of Upstream Bioprocessing are focused on grain handling, milling and hydrolysis of starch into fermentable sugars. This phase encompasses all activities from raw grain material handling and processing resulting in production of suitable fermentation medium. In grain ethanol manufacturing these operations are grouped into specific process steps that are associated with process steps including the following: Corn Receiving, Corn Milling, Slurry Preparation and Liquefaction...              

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FERMENTATION

Alcoholic fermentation is a microbial dependent biocatalytic process where microorganisms such as yeast Saccharomyces cerevisiae convert simple sugars such as glucose, fructose or sucrose into cellular energy, yeast biomass, minor products and ethanol and carbon dioxide as byproduct. Microorganisms of choice for most of the processes are various selected natural or GMM strains of yeast Saccharomyces cerevisiae (commonly referred to as baker’s yeast). Recently the use of genetically modified yeast strains has been allowed in industrial applications. These strains are regulated by their GRASS status and are utilized under MCAN regulations...          

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DOWNSTREAM BIOPROCESSING

Post Fermentation operations are referred to as Downstream Bioprocessing. It is a series of processing and purification steps that occur after the fermentation associated with bioproduction of product, such as ethanol.  Downstream bioprocessing in ethanol manufacturing from corn refers to the series of purification and processing steps that follow fermentation to isolate, purify, and refine ethanol from the crude fermentation mixture (mash) into a high-purity product. It involves separating ethanol from water, solids, and byproducts, ensuring the final product meets quality and regulatory standards for fuel, industrial, or beverage applications...

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